Fig. 3

Staufen1 does not regulate apoptosis in Prostate Cancer cells. Analysis of apoptosis was performed after 48 h of Control (CTL) or Staufen1-shRNA expression (shStau1) in PC cells using Annexin V and Propidium Iodide (PI) dual staining. a Representative western blot of Staufen1 expression with B-actin as a loading control in PC3, DU145, and LNCaP cells. Each representative blot is cropped to show an n = 1 for each cell line from their respective full-length blot. Note that the LNCaP cells used for Fig. 3 experiments were also used for Figs. 1 and 2 and therefore a different representative image from the same blot was selected for Fig. 3. Quantification is represented normalized to CTL (n = 3). b-d Apoptosis was assessed by two-parameter flow cytometry and representative dot plots of CTL and Staufen1-shRNA expressing PC3, DU145, and LNCaP cells. e-g Quantification of Annexin V staining is represented as a percentage relative to CTL (n = 3). Data are Mean ± SD, One-Sample T-Test. Full length blots are presented in Supplemental Fig. 5