Fig. 1

Staufen1 is overexpressed in prostate cancer cell lines. Expression of Staufen1 was determined in human prostate epithelial cells (PrEC), androgen-insensitive (PC3, DU145) and androgen-sensitive (LNCaP) prostate cancer cells. a Western blot using anti-Staufen1 antibodies and GAPDH as a loading control. Both Stau1 isoforms (55 and 63 kDa) were quantified (n = 3). b qRT-PCR analysis using primers specific for Staufen1 mRNA and normalized to total levels of GAPDH (n = 3). Quantifications are normalized relative to PrEC. c Representative western blots using anti-Staufen1 antibodies and B-actin as a loading control (n = 3) in PC3, DU145 and LNCaP cells. Each representative blot is cropped to show an n = 1 for each cell line from their respective full-length blots. Note that the LNCaP cells used for Fig. 1 experiments were also used for Figs. 2 and 3 and therefore a different representative image from the same blot was selected for Fig. 1. Quantification is normalized to CTL (n = 3). d Immunofluorescence of PC3, DU145 and LNCaP cells after 48 h of infection with Control (CTL) or Staufen1-shRNA (shStau1) lentivirus. Cells were stained with an anti-Staufen1 antibody (red) and nuclei with DAPI (blue), scale bars = 50 μm. Data are mean ± SD, Student’s T-Test in (a) and (b), One-sample T-Test in (c) *P < 0.05, **P < 0.01, ***P < 0.001. Full length blots are presented in Supplemental Fig. 3