Fig. 2

MT1JP inhibited proliferation and promoted apoptosis in intrahepatic cholangiocarcinoma cells. A and B. The levels of MT1JP and miR-18a-5p were detected by real-time PCR in normal primary intrahepatic cholangetic epithelial cells and several intrahepatic cholangiocarcinoma cell lines. C. The MT1JP levels were verified in HCCC-9810 and HUCCT1 cells after overexpression or knockdown. D and E. CCK-8 assay was used for detection of cell viability. F. The PCNA expression levels were detected by western blot after ectopic expression or silencing of MT1JP. (The western blot bands were cropped from Fig. S1 and S2.) G. Flow cytometry was used for cell distribution in each phase. H. The expression levels of several cell cycle proteins were detected. (The western blot bands were cropped from Fig. S3, S4, S5 and S6.) I. Flow cytometry was performed for detection of cell apoptosis. J. The expression levels of several apoptosis proteins were examined after MT1JP overexpression in HCCC-9810 cells. (The western blot bands were cropped from Fig. S7 and S8.) (*p < 0.05, **p < 0.01, ***p < 0.001, compared with normal, pcDNA3.1 or siRNA NC; the original images of western blot were shown in supplementary Fig. 1–8)