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Fig. 7 | BMC Cancer

Fig. 7

From: MicroRNA-545 suppresses progression of ovarian cancer through mediating PLK1 expression by a direct binding and an indirect regulation involving KDM4B-mediated demethylation

Fig. 7

Overexpression of KDM4B or PLK1 blocks the inhibitory effect of miR-545 on OC cell growth. a-b mRNA (a) and protein (b) expression of KDM4B and PLK1 in OC cells transfected with miR-545 mimic and the additional oe-KDM4B or oe-PLK1 determined by RT-qPCR and western blot analysis, respectively (relative to GAPDH, two-way ANOVA, compared to miR-545 mimic + oe-NC, *p < 0.05); c viability of cells determined by MTT assay (two-way ANOVA, compared to miR-545 mimic + oe-NC, *p < 0.05); d DNA replication ability of cells measured by EdU labeling assay (two-way ANOVA, compared to miR-545 mimic + oe-NC, *p < 0.05); e proliferation of cells detected by colony formation assay (two-way ANOVA, compared to miR-545 mimic + oe-NC, *p < 0.05); f apoptosis rate of cells measured by TUNEL assay (two-way ANOVA, compared to miR-545 mimic + oe-NC, *p < 0.05); g-h migration (g) and invasion (h) abilities of cells determined by the scratch test and Transwell assay, respectively (two-way ANOVA, compared to miR-545 mimic + oe-NC, *p < 0.05); i angiogenesis ability of HUVECs determined by tube formation assay (two-way ANOVA, compared to miR-545 mimic + oe-NC, *p < 0.05). Representative images are provided. Data were collected from three independent experiments and expressed as mean ± SD

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