Fig. 3

Kpnβ1 knock-down enhances cisplatin sensitivity in cervical cancer cells. a HeLa and SiHa cells were transfected with siRNA for 48 h, after which Kpnβ1 knock-down was confirmed by western blotting, with GAPDH as the loading control. The full-length blots are shown in Supplementary Fig. 3A. b Cisplatin IC₅₀ values were determined in Kpnβ1 knock-down HeLa and SiHa cells and results showed a decrease in cisplatin IC₅₀ in both cell lines when transfected with si-Kpnβ1 compared to si-ctrl. Data shown are results ± SEM (n = 6) of a representative experiment performed two times. c Kpnβ1 knock-down affected cell viability in response to cisplatin treatment in HeLa and SiHa cells. Control and Kpnβ1 knock-down cells were treated with cisplatin for 48 h before viable cells were measured using the MTT assay. Data shown are mean ± SEM (n = 6) of one representative experiment repeated two times (*p < 0.05). d Western blot showing that Kpnβ1 knock-down increased PARP cleavage after cisplatin treatment in HeLa and SiHa cells. GAPDH was included as a loading control, and densitometrical quantification of C-PARP/PARP relative to GAPDH is shown. The full-length blots are shown in Supplementary Fig. 2B. Results are representative of experiments performed two independent times