Fig. 5

Macrophages were treated with EVs from clinical samples. a Morphology and b surface markers of macrophages stimulated by EVs contained in malignant ascitic fluid. Upper figures indicate macrophages differentiated from GM-M0, and lower figures indicate macrophages from M-M0. Scale Bar: 100 μm. c Western blotting for EVs from peritoneal washings of a GC patient with peritoneal nodules and malignant ascites. EVs from Met-5A and GCIY cells were applied for 1.5 μg, and EVs from clinical samples were applied for 1 μl of EV suspension. Full-length blots / gels are presented in Fig. S11. d Western blotting for EVs from peritoneal washings of a GC patient without peritoneal dissemination. EVs from cell lines were applied for 1.0 μg, and EVs from clinical samples were applied for 1.5 μg. Full-length blots / gels are presented in Fig. S12