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Fig. 3 | BMC Cancer

Fig. 3

From: Extracellular vesicles shed from gastric cancer mediate protumor macrophage differentiation

Fig. 3

PBMCs-derived macrophages that were polarized into M2 type by treatment with GCIY-EVs promoted malignancy of cancer cells via IL-6 secretion. a, b The uptake of EVs by day 5-macrophages is visualized. M0-GM Macrophages (a) and M0-M macrophages (b) were stained by Calcein for the cytoplasm, and Hoechst for the nucleus. EVs were stained by PKH26 before co-culture. Magnification, × 200. bar: 100 μm. c Protocol for differentiation of monocytes. d Morphology of M0-GM and M0-M macrophages. e Surface markers of macrophages treated with GCIY-EVs. Upper figures are macrophages treated from M0-GM and lower are from M0-M. CD80 and CD86 were adopted as M1 markers, and CD163 and CD206 as M2 markers. Scale Bar: 100 μm. f Migration assay of MKN7 cells co-cultured with M0-GM macrophages treated by EVs. Control: Cancer cells only. M0-GM: Cancer cells co-cultured with untreated macrophages. M0-GM/MET-EVs: Cancer cells co-cultured with macrophages treated with MET-5A cells-derived EVs. M0-GM/GC-EVs: Cancer cells co-cultured with macrophages treated with GCIY cell-derived EVs. g Migration assay of MKN7 cells co-cultured with M0-M macrophages treated by EVs. h Concentration of IL-6 in the supernatant of macrophages treated with each EV was analyzed by ELISA assay. (*: p < 0.05, NS: not significant, Student’s t-test)

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