Fig. 1

CD163-positive macrophages predominate in both peritoneal washings from a cytology-positive GC patient and malignant ascitic fluid. EVs were contained in malignant ascitic fluid. a and b are immunofluorescent images of the same malignant ascitic fluid. a Cancer cells and monocytes were imaged by the cancer-imaging virus OBP-401 (GFP) and anti-CD14 antibody (PE), respectively. Macrophages were present in malignant ascitic fluid. b M2 macrophages were imaged by anti-CD163 antibody (PE). Scale bar: 100 μm. c Transmission electron microscope image of EVs from MA. Scale bar: 500 nm. d Vesicle size measured by dynamic light scattering analyses. e Representative Western blot of cells and EVs in MA to confirm the presence of EVs by exosome markers (CD9, CD63, CD81). Full-length blots / gels are presented in Fig. S5