Fig. 5

BCOR regulated HOX cluster genes and S100A protein genes. Jurkat cells were transfected with wild-type BCOR or BCOR K607E mutant expressing plasmids, and scrambled siRNA or BCOR siRNA. After 48 h, cells were stimulated with PMA and ionomycin for 6 h for analysis of gene expression. a Hierarchical cluster analysis of HOX and S100A protein expression in wild-type BCOR or BCOR K607E mutant expressing cells revealed the upregulation of several HOX genes and S100A protein genes by BCOR K607E mutant. b and c Quantitative PCR validation of differentially expressed genes selected from the microarray analysis between wild-type BCOR and BCOR K607E mutant expressing cells (b) and fresh-frozen tumor samples (c). GAPDH was used as a control to normalize the levels of these transcripts. Data are shown as the mean ± SEM of five independent experiments performed in triplicates (*P < 0.05, **P < 0.01 compared with cells transfected with wild-type BCOR expressing plasmids and wild-type BCOR tumor samples)