Fig. 7

Immunohistochemical analysis of raptor and rictor expression. a Raptor expression in a representative case of HPV-related OPSCC with high raptor expression in real-time PCR (∆∆CT = 4.29). Strong raptor immunoreactivity was also observed in the cytosol of cancer cells. Bar, 100 μm. b Rictor expression in a representative case of HPV-related OPSCC with high rictor expression in real-time PCR (∆∆CT = 5.24). The sample was obtained from the same patient as in a. Strong rictor immunoreactivity was also observed in the cytosol of cancer cells. Bar, 100 μm. c Raptor expression in a representative case of HPV-related OPSCC with low raptor expression in real-time PCR (∆∆CT = 0.81). Weak raptor immunoreactivity was also observed in the cytosol of cancer cells. Bar, 100 μm. d Rictor expression in a representative case of HPV-related OPSCC with low rictor expression in real-time PCR (∆∆CT = 1.29). The sample was obtained from the same patient as in c. Weak rictor immunoreactivity was also observed in the cytosol of cancer cells. Bar, 100 μm. e Raptor expression in a representative case of HPV-unrelated OPSCC with high raptor expression in real-time PCR (∆∆CT = 3.37). Strong raptor immunoreactivity was also observed in the cytosol of cancer cells. Bar, 100 μm. f Rictor expression in a representative case of HPV-unrelated OPSCC with high rictor expression in real-time PCR (∆∆CT = 2.29). The sample was obtained from the same patient as in e. Strong rictor immunoreactivity was also observed in the cytosol of cancer cells. Bar, 100 μm. g Raptor expression in a representative case of HPV-unrelated OPSCC with low raptor expression in real-time PCR (∆∆CT = 1.03). Weak raptor immunoreactivity was also observed in the cytosol of cancer cells. Bar, 100 μm. h Rictor expression in a representative case of HPV-unrelated OPSCC with low rictor expression in real-time PCR (∆∆CT = 0.75). Weak rictor immunoreactivity was also observed in the cytosol of cancer cells. Bar, 100 μm