Fig. 5
Effects of incubation with temsirolimus. a Cell survival ratios in the WST-1 assay after 24-h incubation with various concentrations of temsirolimus. Although the cell survival ratios of each cell line were slightly different, ≥12 μM temsirolimus inhibited cell survival in all cells. A prominent difference in cell survival among the cell lines was observed at 6 μM temsirolimus. b Box plots of cell survival rates in the cell lines after incubation with 6 μM temsirolimus for 24 h. The cell survival ratios of the UM-SCC47 and CaSki cell lines were significantly worse than those of the SCC-4 and SAS cell lines. *p < 0.05; **p < 0.01. c Temsirolimus induces cell cycle arrest in the UM-SCC47 and CaSki cell lines. UM-SCC47, CaSki, and SCC-4 cells were arrested at the G0/G1 and G2/M phases by incubation with temsirolimus and the number of cells in the S phase was significantly reduced in the UM-SCC47 and CaSki cell lines. In contrast, only SAS cells showed a decrease in the number of cells in the G2/M phase. *p < 0.05; **p < 0.01. The original flow cytometry data are provided in Additional file 3