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Fig. 4 | BMC Cancer

Fig. 4

From: Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p/RAI14 axis

Fig. 4

AFAP1-AS1/miR-653-5p/RAI14 axis is involved in melanoma progression. a qRT-PCR analyzed RAI14 mRNA expression in pcDNA3.1 or pcDNA3.1/RAI14-transfected cells. b Western blot assay revealed the expression of RAI14 in pcDNA3.1 or pcDNA3.1/RAI14-transfected cells. c CCK-8 assay reflected cell proliferation at indicated times in transfected cells. d Colony formation assay was performed to disclose colongenic ability of transfected cells. e Cell migration in different groups was observed in transwell assay. f Transwell assay explored cell invasion. g Western blot assay was utilized to measure the expression of EMT process related proteins. *P < 0.05, **P < 0.01

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BMC Cancer

ISSN: 1471-2407

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