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Fig. 2 | BMC Cancer

Fig. 2

From: Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p/RAI14 axis

Fig. 2

AFAP1-AS1 directly binds to miR-653-5p and represses miR-653-5p expression. a FISH assay was used to confirm the location of AFAP1-AS1 in A375 cells. b 29 miRNAs were screened out by ENCORI (The Encyclopedia of RNA Interactomes) website to have binding sites for AFAP1-AS1. qRT-PCR assay detected the up-regulation of miRNAs (miR-653-5p, miR-370-5p, miR-195-5p, miR-497-5p and miR-512-3p) by AFAP1-AS1 knockdown. c The binding ability of miRNAs to AFAP1-AS1 was verified through RNA pull down assay. d The expression of miR-653-5p in melanoma cells was measured by qRT-PCR assay. e qRT-PCR assay explored the overexpression and knockdown efficiency of miR-653-5p. f qRT-PCR assay investigated the relationship between the expression level of AFAP1-AS1 and miR-653-5p. g There were the binding sites between AFAP1-AS1 and miR-653-5p. h Luciferase reporter assay was conducted to verify the binding ability between AFAP1-AS1 and miR-653-5p. i-j CCK-8 and colony formation assay disclosed cell proliferation. k-l Cell migration and invasion were observed in transwell assays. m Western blot assay was applied to measure the expression of EMT process related proteins. *P < 0.05, **P < 0.01

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