Fig. 3

Evaluation of CX-4945-induced effects on BACH2 and BCL6 gene and protein expression in SEM cells. SEM cells were cultured and incubated with 5 μM CX-4945 or DMSO (control) for up to 96 h. a Changes in BACH2 and BCL6 gene expression were assessed by qRT-PCR after 48 h and 72 h. Mean values of ΔCT values from DMSO-treated control cells were calculated and set to 1 for each time point. ΔΔCT values were calculated for CX-4945-treated samples and compared to the respective time-matched control. Analyses were carried out in three independent biological and technical replicates. Mean ± standard deviation; * p < 0.05. b, c Analysis of BACH2 and BCL6 protein expression as well as AKT phosphorylation was carried out by western blot with GAPDH as housekeeping gene. Representative images of three independent biological experiments. Blots were processed and cropped using Image Studio Lite 5.2 software and MS PowerPoint (2011) to improve clarity and conciseness. Full size blots are uploaded in Additional File 8: Fig. S5. b Short term effects of CX-4945 incubation were determined after 0.5 h, 2 h and 6 h. c Long term effects of CX-4945 incubation were determined after 24 h, 48 h, 72 h and 96 h