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Fig. 3 | BMC Cancer

Fig. 3

From: Unphosphorylated STAT3 in heterochromatin formation and tumor suppression in lung cancer

Fig. 3

STAT3 and HP1α affect cellular senescence and major satellite transcription. (a) Representative images are shown of A549 cells stably expressing the indicated STAT3 or HP1α transgenes assayed for senescence-associated beta-galactosidase (SA-β-gal; blue) at pH 6.0 [53]. Control cells were parent A549 cells without transgene expression. (b) Cell senescence was quantified by measuring senescence-associated β-Gal staining at an absorbance of 405 nm. (c) Total RNA was isolated from A549 cells stably expressing the indicated transgene, and were quantified for major satellite transcripts using qRT-PCR. GAPDH gene transcript was used as the internal control for expression. The mean value ± s.d. (standard deviation) was calculated in each group. * P < 0.05, ** P < 0.01

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