Fig. 1From: The kinetic profile and clinical implication of SCC-Ag in squamous cervical cancer patients undergoing radical hysterectomy using the Simoa assay: a prospective observational studySimoa SCC-Ag assay calibration curve and validation. a Typical Simoa SCC-Ag assay calibration curve. Recombinant human SCC-Ag was serially diluted, and the calibrator range was 0.049 to 50 ng/mL with a recovery of all back-calculated concentrations between 80 and 120%. The fitting model for the calibration curve was a weighted four-parameter logistics (1/Y2). AEB: Average enzyme per bead (measured signal). b Validation results and acceptance criteriaBack to article page