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Fig. 3 | BMC Cancer

Fig. 3

From: EGFR modulates complement activation in head and neck squamous cell carcinoma

Fig. 3

Complement activation after EGFR inhibition is C1q dependent. (a) Immunofluorescence microscopy of HN5 cells shows deposition of C4 (green) on surface of Iressa treated cells after incubation with NHS, but not HIS. Controls are not treated with Iressa but incubated with NHS, C4 fluorescence was quantified in the bar chart to the right (b) Immunofluorescence microscopy of HN5 cells show no deposition of complement activation fragments C3 (green) and terminal complement complex TCC (red) on surface of HN5 cells after 48 h of Iressa treatment and subsequent incubation in C1q depleted serum, or C1q only (100 μg/mL), but when C1 depleted serum was reconstituted with C1q (100 μg/mL), complement activation was restored. While incubation with Factor B depleted serum, lacking ability to form alternative pathway C3 convertase, still shows complement activation, C3 and TCC fluorescence was quantified in the bar chart to the right. Scale bars, 10 μM. Experiment was repeated three times

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