Fig. 4
Vemurafenib and afatinib produce additive growth-limiting effects in CRC BRAFV600E cell lines. Cells were exposed to VEM (3 μM), AFA (1 μM or 10 μM), or both (VEM + AFA1, VEM + AFA10, respectively) for 48 h, harvested, and assayed for (a, c, e) cell viability and (b, d, f) cell death. Results are means ± SD of three independent experiments. Statistics (two-way ANOVA for multiple comparisons): *p < 0.05, ** p < 0.001 vs. CTRL (untreated cells); ° p < 0.05, °° p < 0.01 vs. cells treated with the indicated drug or drug combo (horizontal bars)