Fig. 3

Fluorescence imaging with EP-HMRG of adenocarcinoma with a background of intestinal metaplasia located at the G (case #5) and E with LSBE (case #7). a Endoscopic imaging with white light before endoscopic submucosal dissection (ESD). b Endoscopic imaging with white light after ESD. c Fluorescence imaging after EP-HMRG spraying. d Resected specimen mapping for the tumor region. The adenocarcinoma is shown as red lines. e Time course of the fluorescence intensity of the tumor lesion and the non-tumor region after EP-HMRG spraying. f Hematoxylin and eosin staining and immunohistochemical examination investigating DPP-IV expression of the tumor lesion and non-tumor region. g Immunohistochemical examination investigating the subtype of intestinal metaplasia in the non-tumor region. A decrease in the expression of MUC5AC and MUC6 and the positive expression of MUC2 and CD10 indicate complete intestinal metaplasia (#5). The positive expression of MUC5AC, MUC6, and MUC2 and negative expression of CD10 indicates incomplete intestinal metaplasia (#7). Scale bars of (b–d), 5 mm. Scale bars of (f and g), 200 μm