Fig. 4

Verteporfin is cytotoxic for HR⁻ BCa and PCa cell lines. (a) MDA-MB-231, BT549, PC3, and DU145 cell lines were treated with vehicle control or 10 μM verteporfin for 1 day. Western blot analysis shows oligomerized p62 (p62*), indicating treatment efficacy. (b) Representative images of DU145 cells treated with vehicle control or 5 μM verteporfin for 7 days and immunostained for p62 (FITC; nuclei, DAPI; 40X magnification, scale bar = 50 μm). Verteporfin induces p62 oligomerization indicated by p62 puncta. Cells were treated with vehicle control or 10 μM verteporfin for 5 days and (c) MTT assay was performed to assess cell viability or (d) cell counts were taken at day 0 (no treatment), day 1, 3, and 5. Verteporfin is cytotoxic for MDA-MB-231, BT549, PC3, and DU145 cell lines. N = 3 biological replicates; error bars, +/−STDEV; p-value, ** ≤ 0.005, *** ≤ 0.0005. Cell viability is normalized to vehicle control for each cell line and cell counts are normalized to day 0 for each cell line