Fig. 2

RT-qPCR validation of select genes from 350 gene signature. RT-qPCR was performed for select genes for LNCaP and MCF7 cell lines treated with vehicle control or 25 ng/ml IL-1α or IL-1β for 3 days (LNCaP) or 5 days (MCF7). MDA-MB-231, BT549, PC3 and DU145 cell lines were treated with vehicle control only. RT-qPCR is shown for the BCa (a) and PCa (b) cell lines. RT-qPCR shows that CCL20, CD68, IL8, p62, SOX9 and Zeb1 are induced by IL-1 in MCF7 and/or LNCaP cell lines and are basally high in MDA-MB-231, BT549, PC3 and/or DU145 cell lines. RT-qPCR shows that CXCR7 and MMP16, but not CDK2 or PLK1, are downregulated by IL-1 in MCF7 and/or LNCaP cell lines and are basally high in MDA-MB-231, BT549, PC3 and/or DU145 cell lines. N = 3 biological replicates; error bars, +/−STDEV; p-value, * ≤ 0.05, ** ≤ 0.005, *** ≤ 0.0005. mRNA fold change is normalized to MCF7 vehicle control for the BCa cell lines and to LNCaP vehicle control for the PCa cell lines