Fig. 6

Inhibition of ELTD1 reduced optic nerve invasion in the aggressive Rb phenotypic cell line. A Schematic images illustrate procedure prior to Rb cell inoculation. Co-immunocompromised mice were weighted and intraocular pressure (IOP) was measured prior to and after Rb cell inoculation (controls and Y79 transfected with siELTD1). After mice reached an IOP ≥15 mmHg, closely near 2 months’ post-inoculation, mice were enucleated, and eyes for removed for histological analysis. B, B1-B3 Controls untransfected Weri-Rb-1 cells show intraocular invasion, without extraocular involvement. C, C1-C2 Eyes inoculated with transfected siELTD1 Weri-Rb-1 cells displayed similar invasion patterns as their counterpart control conditions (B). D, D1-D3 Controls untransfected Y79 cells show intraocular invasion, with extraocular optic nerve involvement. E, E1-E3 Eyes inoculated with transfected siELTD1 Y79 cells displayed substantial reduction in optic nerve invasion. Objective 4x was used for images B, B1, C, D and E while 10x was used for images B2-B3, C1-C2, D1-D3 and E1-E2. AC: Anterior Chamber; ON: Optic Nerve; VC: Vitreous Cavity