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Fig. 5 | BMC Cancer

Fig. 5

From: Adhesion G protein-coupled receptor, ELTD1, is a potential therapeutic target for retinoblastoma migration and invasion

Fig. 5

GPR125 does not play a role in Rb cell migration. a-l Subsequent to GPR125 silencing, control untransfected (UT) cells (Y79: A-C; Weri-Rb-1: G-I), siGPR125 transfected Y79 (D-F) and siGPR125 transfected Weri-Rb-1 cells (J-L) were we applied in serum free media in an upper chamber of an 8 um polycarbonate membrane. Cells were left incubated for 6 h to enable migration as stimulated by the supplemented media in the lower chamber. Migrating cells were fixed and stained with Hoechst dye for nuclear stain to ensure that quantification of blue migrating cells overlapped with the membrane’s pores (white arrowhead). m Quantitative analysis show a significant increase in migration of UT Y79 cells compared to UT Weri-Rb-1 cells, as expected. However, no reduction in migration was observed in the Rb cells after GPR125 silencing. n. Silencing of GPR125 did not have an effect in Rb cell viability. Ordinary One-Way ANOVA followed by Tukey’s multiple comparison test (M and N) was used to compare between columns. Scale bar for panels A-L is 10 μm. Error bars, SEM. Significance was expressed as **p < 0.01

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