Fig. 4

ELTD1 Silencing significantly reduced cell migration in Y79 Rb cells. a-l Rb cell lines were transfected with HiPerfect Reagent to induce silencing of ELTD1. Control untransfected (UT) cells (Y79: A-C; Weri-Rb-1: G-I), siELTD1 transfected Y79 (D-F) and siELTD1 transfected Weri-Rb-1 cells (J-L) were cultured in serum free media in the upper chamber of an 8 um polycarbonate membrane to measure cell migration. Cells were incubated for 6 h to enable migration to the bottom chamber by provision of complete media. Migrating cells were fixed and labeled with Hoechst dye to enable quantification of migrating cells (white arrowhead). m Quantitative analysis show a significant reduction in migration of siELTD1 cells compared to UT cells. This effect was specific to Y79 cells, as Weri-Rb- 1 cell migration was unaffected. n Silencing of ELTD1 did not have an effect in Rb cell viability. Ordinary One-Way ANOVA followed by Tukey’s multiple comparison test (M and N) was used to compare between columns. Scale bar for panels A-L is 10 μm. Error bars, SEM. Significance was expressed as ***p < 0.001 and ****p < 0.0001