Fig. 2

Western blot analysis show overexpression of ELTD1, but not GPR125, in Rb human tumors compared to fetal retina samples. a, c Immunoblot illustrate specificity of ELTD1 (77 kDa) and GPR125 (146 kDa) antibodies, respectively. For ELTD1 antibody specificity, human brain and HeLa cell lysates were used as negative and positive controls, respectively. For GPR125 antibody specificity, Jurkat and HeLa cell lysates were used as negative and positive controls, respectively. b Quantitative analysis show a significantly higher ELTD1 protein expression in Rb cells as well as in Rb tumors compared to fetal retina controls. d Quantitative analysis show a significantly higher GPR125 protein expression in Rb cells, Weri-Rb-1 and Y79, but not in Rb tumors compared to fetal retina controls. GAPDH (37 kDa) antibody was utilized as a loading control. Images shown are cropped from full-length blots presented in Additional File 2: Supplementary Figure 1, to improve the clarity and conciseness of the data presented. Relative protein expression in blot images was analyzed with Image Studio Lite Version software (version 5.2.5). Two-tailed Student’s t test (A and C) and Ordinary One-Way ANOVA followed by Tukey’s multiple comparison test (B and D) were used to compare each column with their control counterpart. Error bars, SEM. Significance was expressed as: * p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001