Fig. 6

AGR2 does not affect EGFR expression and activation in PANC-1 cells. a The stable cells were treated with 20 μM gemcitabine (Gem) for the indicative times, and the levels of total EGFR protein were determined by Western blotting. b Flow cytometry was performed to assess levels of cell surface EGFR in untreated and Gem-treated cells. The signal intensity is plotted on the abscissa and the number of events on the ordinate. c The cells were treated with gemcitabine for the indicative times, and collected cell lysates were examined for the phosphorylated EGFR by Western blotting