Fig. 4

AGR2 promotes PANC-1 cell survival under ER stress induced by tunicamycin (Tm). a Colony assay was used to determine cell survival in the absence or presence of tunicamycin (1 μg/mL). b Tunicamycin-induced cell apoptosis was assessed by flow cytometry with the co-staining of PI and Annexin V. *, p < 0.01 vs. Vector. c PANC-1 stable cells were treated with tunicamycin (1 μg/mL) for the indicated times, and whole cell lysates were analyzed by Western blotting against cleaved Caspase-3 with GAPDH as loading control. d PANC-1 stable cells were treated with tunicamycin (2 μg/mL) for the indicated times, and the levels of CHOP were detected by Western blotting. GAPDH levels were measured as loading control