Fig. 1

Generation of PANC-1 cells stably expressing ER-resident and secreted AGR2. a The primary structure of the AGR2 protein shows identified functional domains and motifs. b Expression levels of AGR2 mRNA in PANC-1 stable cell lines were detected by RT-PCR. c Western blotting was used to analyze the presentation of the AGR2 protein in whole cell lysates of PANC-1 stable cell lines. GAPDH served as a loading control. d The presence of AGR2 proteins in cell lysates and culture supernatant was detected by Western blotting. e DSP-stabilized AGR2 in cell lysates and culture supernatant was analyzed under non-reducing (top blot) or reducing conditions (bottom blot). f Cellular distribution of AGR2 and ER-associated protein, calnexin, were determined by immunofluorescence. AGR2 is shown in red, calnexin in green, and Hoechst 33342 in blue. Scale bars: 20 μm