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Fig. 2 | BMC Cancer

Fig. 2

From: Transcriptome profiling unveils GAP43 regulates ABC transporters and EIF2 signaling in colorectal cancer cells

Fig. 2

Silencing of GAP43 gene is due to aberrant promoter methylation. a SW620 cells were treated with the indicated concentration of DNA demethylating agent 5-Aza-CdRfor 72 h, or b SW620 cells were treated with 10 μM 5-Aza-CdR for indicated treatment time (0, 24, 48, 72 h), and then mRNA levels of GAP43 were analyzed by qRT-PCR. c Bisulfite genomic sequencing was performed to analyze the methylation status of the GAP43 promoter in CRC cell lines SW620 and HCT116. Multiple CpG islands in the promoter region of the GAP43 gene were analyzed. The results represent at least three independent experiments and are reported as mean ± SEM. *p < 0.05, **p < 0.001

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