Fig. 5

Immunohistochemical analysis in the pancreatic peritoneal dissemination model treated by Amatuximab. Immunohistochemistry was performed on indicated sections from Amatuximab or control treated model mice for proteins related with a chemoresistance, c epithelial mesenchymal transition, adhesion, d, f stemness and metastasis. b, e Quantification of positively stained cells for the indicated proteins. Arrows indicate the representative image of the positively stained cells. The frequencies of positively stained cells were counted in six high power fields that were chosen at random. g Quantitative PCR of the indicated factors in RNA samples harvested from blocks. *P < 0.05. Scale bar, 100 μm