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Fig. 1 | BMC Cancer

Fig. 1

From: Mesothelin blockage by Amatuximab suppresses cell invasiveness, enhances gemcitabine sensitivity and regulates cancer cell stemness in mesothelin-positive pancreatic cancer cells

Fig. 1

Comparison of mesothelin expression in human pancreatic cancer cell lines. a Whole cell lysates of AsPC-1, Capan-2, Panc-1 and MIA Paca-2 cells were analyzed for mesothelin expression by western blotting. β-actin was used as an internal control. Full-length blots/gels are presented in Supplemental Figure 2. Densitometric analysis of western blots was performed using a ChemiDoc XRS Plus system with Image Lab Software (Bio-Rad, Hercules, CA, USA). b FACS analysis of mesothelin expression on the surface of the four human pancreatic cancer cell lines. Onecomp eBeads (#01–1111, eBioscience) is used for positive control and FITC Mouse IgG1κ Isotype Control (#555748, BD Biosciences) is used for negative control. The results of analysis of mesothelin expression in the four human pancreatic cancer cells by immunocytochemistry were shown in Supplementary Figure 2

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