Effects of EHHADH knockdown on cell proliferation, migration, invasion, and apoptosis in CDDP-R BC cells. a EHHADH mRNA expression [measured by RT-qPCR] and (b) EHHADH protein levels [measured by Western blot analyses] were assessed to validate the knockdown efficiency of si-EHHADH. *, P < 0.001. c Cell proliferation determined by XTT assay. *, P < 0.001. The full-length blots/gels are presented in Supplementary Figure 8. d Cell invasion activity determined with the Matrigel invasion assay. *, P < 0.001. e Cell migration activity determined with wound healing assays. *, P < 0.001; **, P < 0.01. All experiments were performed in quadruplicate and si-EHHADH transfectants were compared with mock or si-control transfectants. f Western blot analysis of apoptotic markers (cleaved PARP and PARP) in CDDP-R-BOY and CDDP-R-T24. The full-length blots/gels are presented in Supplementary Figure 8.