Fig. 1

Collagen F/B is heterogeneous within primary breast tumor tissue. SHG F/B images (a series of adjacent ROIs extending along the x-axis) and matching H&E images were stitched end-to-end to form a composite ROI for a representative sample. Examples of tumor bulk (solid box), tumor-stroma interface (dashed box), and uninvolved tissue (circle) are shown. The F/B values were plotted in a false color “heatmap” (low F/B values = dark blue, high F/B values = yellow) to illustrate differences in F/B within the tumor region including tumor bulk and tumor-stroma interface, indicating intratumoral heterogeneity in F/B