Fig. 2

MiR-383 inhibits translation of Bcl-2 mRNA via 3′-UTR binding. a Bioinformatic prediction. b-c Overexpression or depletion of miR-383 in 2 human GC cell lines, AGS (b) and SNU-5 (c), by transfection with a miR-383-mimic, or with a plasmid carrying miR-383 antisense. Transfection with a null plasmid was used as a control. RT-qPCR was performed to assess miR-383 levels. For B and C, values were normalized to null-transfected cells (=1). (D-E) MiR-383-modified AGS (d) or SNU-5 (e) cells were transfected with wildtype or mutant Bcl-2-3’UTR for a luciferase reporter assay. For D and E, values were normalized to null only -transfected cells (=1). *p < 0.05. N = 5 (each experimental group contains 5 repeats)