Fig. 4
From: Semaphorin 3A mediated brain tumor stem cell proliferation and invasion in EGFRviii mutant gliomas
Sema3A pro-invasive effects are Nrp1 and PlxnA1 dependent. Gap migration assay with Control, Nrp1-KD and PlxnA1-KD BTSCs in the absence and presence of Sema3A (100 ng/mL). Control non-targeting virus treated BTSCs increase invasive migration in response to Sema3A. Nrp1-KD and PlxnA1-KD BTSCs are unresponsive to Sema3A. Nrp1-KD cells demonstrate an increased baseline invasive index (**p < 0.005; ***p < 0.0005; NS, not significant)