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Fig. 2 | BMC Cancer

Fig. 2

From: Semaphorin 3A mediated brain tumor stem cell proliferation and invasion in EGFRviii mutant gliomas

Fig. 2

Sema3A drives invasion of BTSCs. a Gap migration assay demonstrating increased percentage of cells within initial boundaries (white dashed line), indicating increased invasive migration in Sema3A (100 ng/mL) treated BTSCs compared to Control over 24 h (green = phalloidin; scale bar = 100 μm). b Quantification of gap migration assays showing increased invasive index with Sema3A treatment (***p < 0.0005). c 3-dimensional Matrigel tumorsphere invasion assays comparing Control and Sema3A (100 ng/mL) treated tumorspheres for 12 h, showing increased process extension with treatment, indicating increased invasion (scale bar = 200 μm). d Quantification of 3-dimensional invasion assay showing increased invasion index with Sema3A treatment compared to Controls (*p < 0.05). e BrdU labeling of Control and Sema3A (10 ng/mL) treated BTSCs demonstrating decreased BrdU positive cells (green) as well as decreased total cells (blue) per field (scale bar = 50 μm). f Quantification of proliferation assays in mean BrdU positive labeled cells per field (mpf) (**p < 0.005). g PI labeling of Control and Sema3A treated BTSCs demonstrating no difference in percent positive cells, indicating no change in cell death

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