Fig. 4

Ectopic overexpression of MNAT1 promotes OS cell proliferation and invasion in vitro. U2OS and 143B cells were transfected with MNAT1 plasmid or negative control (NC). a The mRNA expression of MNAT1 was examined by qRT-PCR. b The transfection efficiency was evaluated by western blot assay and EdU staining assays (Scale bars, 50 μm) (c) and colony formation assays (Scale bars, 8 mm) (d) were performed to determine the proliferation of U2OS and 143B cells. e Cell migration was assessed by wound-healing assay (Scale bars, 500 μm). f Cell invasion was assessed by transwell assay (Scale bars, 50 μm). *p < 0.05, **p < 0.01