Gene expression analysis of human prostate cell lines with and without tumor metastasis suppressor CD82

BMC Cancer

Table 1 qRT-PCR primers for validating microarray gene expression

	Primer orientation	Primer sequence (5′-3′)	Melting Temperature (Tm⁰C)	GC%
RUNX3	Forward primer	GACAGCCCCAACTTCCTCT	56.9	57.8
RUNX3	Reverse primer	CACAGTCACCACCGTACCAT	57.0	55
TFF3	Forward primer	TCAAGCCCCTGCAGGAAGCAG	62.4	61.9
TFF3	Reverse primer	GCCGGGAGCAAAGGGACAGA	62.4	65
β-actin	Forward primer	AGCACTGTGTTGGCGTACAG	57.9	55
β-actin	Reverse primer	CTCTTCCAGCCTTCCTTCCT	56.4	55

ISSN: 1471-2407