Fig. 5

LAD1 contributes to liver metastasis of SW620 cells in vivo. a LAD1 in SW620 cells was stably knocked down by shRNA. The levels of LAD1 RNA in cells depleted of LAD1 (shLAD1) or control cells infected with virus carrying scramble RNA (shSCR) were determined by RT-PCR. Shown are the cropped gel image. b The liver weights of mice injected with SW620 cells depleted of LAD1 (shLAD1, n = 3) were comparable with those of the control (shSCR, n = 3). c, d Livers from shLAD1 mice carried fewer tumor nodules (marked by arrowheads) than those from shSCR mice. Representative images of metastatic nodules in mouse livers with scale bars for 1 cm are shown in c. The number of metastatic nodules in the liver is shown in (d). e H&E staining and immunohistochemistry with anti-Ki67 antibody showed that the nodules in the liver were tumorigenic. Scale bars denote 200 μm