Fig. 4

Effect of stimulation of B7-H6 on tumor cell migration. Using the scratch method to approximate cell migration or wound healing, the effect of B7-H6 stimulation was evaluated for 72 h. In (a) and (c): photographs of unsorted HeLa and SiHa cells migrating across cell free “wounds” in culture. Cells were incubated with 0–80 ng/mL of sNKp30 and compared with cells incubated with media (basal) or cisplatin (1 μg/ml), a control for the inhibition of migration. In (b) and (d): graphical representation of the resulting changes in scratch distance (wounds) from three independent experiments with HeLa and SiHa cells, respectively; *p < 0.05 (analysis of variance)