Table 2 Summary of immunohistochemistry procedures used in this study
Antibody | Antigen retrieval treatment | Supplier | Dilution | Clone | Control |
|---|---|---|---|---|---|
Ki-67 | PT Link (97 °C, 20 min) Target Retrieval Solution High PH | DAKO, Denmark | Ready to use | MIB-1 | Epithelial cell |
GLUT2 | AC (121 °C, 5 min), pH 6.0 | proteintech, USA | 1:500 | polyclonal | Islet of langerhans |
MGMT | AC (121 °C, 5 min), pH 6.0 | Millipore, USA | 1:200 | MT3.1 | Vascular endothelial cell |