Fig. 2
EP300 KD reduces tumorspheres formation. a, Tumorsphere assay (14 days in culture) of MDA-MB-231EP300KD and scramble clones (n = 3 per condition). b, Tumorsphere assay (7 days in culture) after transient EP300 KD (48 h) in BT20 cells (n = 3 per cell line per condition) (scale bar 125 μm). (*p < 0.05, **p < 0.01). C, Gene expression analysis of CSC markers (EP300, ABCG2, ABCC1, NES) in scramble tumorspheres and EP300 KD (clone 2) tumorspheres (n = 3 per cell line)