Fig. 1

GTF2I-ABCB1 fusion induced by daunorubicin exposure of THP-1 AML cells identified by nanopore sequencing. a RNAseq and H3K27Ac ChIPseq tracks for the indicated cell lines surrounding ABCB1 (upper left panel) and the promoter of GTF2I (upper right panel). Middle left panel: local contact 4Cseq profile of THP-1_S and THP-1_R cells using an ABCB1 promoter centered viewpoint. Lower panels show chimeric reads generated by targeted nanopore sequencing of the ABCB1 locus. In each region the breakpoint is highlighted in blue. Pink or blue coloring of reads indicates orientation. b 4Cseq contact profile for a 17 Mb region of chromosome 7. c Tracks show the 500 kb region surrounding ABCB1 targeted for sequencing, the region expected to be covered by RNA hybrid capture and the coverage achieved using conventional sequencing (MiSeq) and nanopore long-read sequencing (MinION) of THP-1_R cells. Bottom panel shows RNAseq tracks from THP-1_S and THP-1_R cells