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Fig. 5 | BMC Cancer

Fig. 5

From: Bladder cancer-derived interleukin-1 converts the vascular endothelium into a pro-inflammatory and pro-coagulatory surface

Fig. 5

Microfluidic experiments indicated the pro-inflammatory and pro-coagulatory activation of endothelial cells by tumour cell SN. HUVECs were perfused at 6 dyn/cm2 with hirudinated whole blood for 15 min. a, b In comparison to the SN of UROtsa cells, pre-treatment of HUVECs with T24 cell SN for 6 h promoted platelet binding (green) to gaps between the endothelial cells (white). CD31 was used as endothelial marker, vWF was used to identify platelets. (Additional experiments performed with washed and fluorescent platelets are shown in the data supplement.) b Magnified region of platelets bound to HUVECs treated withT24 cell SN. Quantitative analysis of the platelet covered area is shown as bar diagram. n = 3; * P ≤ 0.05 a, c Pre-treatment of HUVECs with T24 cell SN mediated the recruitment of leukocytes (red). c Magnified region of leukocytes attached to HUVECs treated withT24 cell SN. CD45 was used as leukocyte marker. The number of recruited leukocytes is shown as bar diagram; n = 3; * P ≤ 0.05. a-c Nuclei (blue) were stained with DAPI, scale bars correspond to 50 μm

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