Fig. 7

DLC-1 inhibits the activity of the Notch1-Hey1 axis, but not the Notch1-Hes1 axis, in the immunomodulation of hUC-MSCs. a RT-PCR validated that the transfection with siHes1 (1#) and siHey1 (1#) effectively inhibited the gene expression of Hes1 and Hey1, respectively. b The IDO1 promoter activity assay indicated that, comparing with siControl, the siHes1 transfection caused a slight increase, but the siHey1 transfection resulted in a dramatic decrease, in the IDO1 promoter activity. c The Th1 lymphocyte proliferation assay showed that the transfection with siHey1, but not siHes1, was able to significantly reduce the inhibition of Th1 proliferation by hUC-MSCs. d RT-PCR showed that the treatment with GSI-I equally reduced the gene expression of both Hes1 and Hey1, whereas DAPT only reduced that of Hes1. e Western blotting showed that DAPT caused a slight increase in, but GSI-I resulted in a significant decrease in, the IDO1 expression. f The Th1 lymphocyte proliferation assay indicated that GSI-I reduced, but DARP exhibited no effect on, the inhibition of Th1 lymphocyte proliferation by hUC-MSCs