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Fig. 6 | BMC Cancer

Fig. 6

From: The DLC-1 tumor suppressor is involved in regulating immunomodulation of human mesenchymal stromal /stem cells through interacting with the Notch1 protein

Fig. 6

A mutual exclusion relationship exists between DLC-1 and Notch1 in hUC-MSCs. Western blotting showed that the siDLC-1 transfection reduced both basal and GSI-I-induced NICD1 (a); the transfection with DLC-1 cDNA caused a significant reduction of basal NICD1 level (b); in the co-transfection studies, whereas the transfection with NICD1 cDNA alone showed an over-expression of NICD1 and increases, and the transfection with DLC-1 cDNA alone reduced, the IDO1 expression, the co-transfection of both cDNAs inhibited NICD1 over-expression and the increased expression of IDO1 (c); in analyzing the partition in cellular compartments of DLC-1 and NICD1 proteins, the siDLC-1 transfection clearly increased the NICD1 level in both the cytoplasm and the nucleus, whereas the DLC-1 cDNA transfection reduced it in both cellular compartments with a more significant reduction seen in the nucleus than the cytoplasm (d). In all the experiments, the siControl and pcDNA3.1 were used as control in siRNA transfection and plasmid DNA transfection, respectively

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