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Fig. 5 | BMC Cancer

Fig. 5

From: The DLC-1 tumor suppressor is involved in regulating immunomodulation of human mesenchymal stromal /stem cells through interacting with the Notch1 protein

Fig. 5

The effect of DLC-1 in hUC-MSCs is achieved through inhibiting the Notch signaling. A In the IDO1 promoter assay from the co-trasnsfection of DLC-1 cDNA with siNotch1, the Notch1 silencing was confirmed by Western blotting (A-1). While DLC-1 alone or siNotch1 alone reduced, the co-transfecton further inhibited, the IDO1 promoter activity (A-2). B In the IDO1 promoter assay from the co-trasnsfection of DLC-1 cDNA with NICD1 cDNA, the NICD1 over-expression was confirmed by Western blotting (B-1). DLC-1 inhibited the NICD1-elevated IDO-1 promoter activity (B-2). The IDO1 promoter activity was measured from the transfection with pIDO1-Luc followed by the treatment with IFN-γ

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