Fig. 6

Effects of trastuzumab and afatinib on cell motility and average speed. MKN1 (a, b), MKN7 (c, d) NCI-N87 (e, f) and Hs746T (g, h) cells were treated with 5 μg/ml trastuzumab (Tra), 0.5 μM afatinib (Afa), 5 μg/ml trastuzumab + 0.5 μM afatinib (Tra + Afa) or afatinib solvent DMSO (0.05%). Untreated (untr) cells were used as control. Time-lapse microscopy was carried out for 7 h. Cell movement was tracked to assess motility (a, c, e, g) and average speed (b, d, f, h). Shown are the mean values of three to six experiments with standard deviation. Statistically significant effects are indicated by *p < 0.05 or **p < 0.01 (two-sample t-test)