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Fig. 5 | BMC Cancer

Fig. 5

From: Determining the effects of trastuzumab, cetuximab and afatinib by phosphoprotein, gene expression and phenotypic analysis in gastric cancer cell lines

Fig. 5

Effects of trastuzumab and afatinib on kinase phosphorylation in NCI-N87 cells. The effect of trastuzumab and/or afatinib on several protein tyrosine kinases in NCI-N87 cells was analyzed by Luminex technology. Cells were treated for 3, 5, 15, 30, 60 and 240 min with 0.5 μM afatinib, 5 μg/ml trastuzumab or the combination of both. In the batch-corrected cluster analysis, the x-fold change of each activated protein is shown. Samples were clustered based on the similarity of the activated proteins and treatment conditions. Significant effects between different treatment conditions are indicated by (*) with increasing size (0.01 < p-value ≤0.05, 0.001 < p-value ≤0.01 and p-value ≤0.001). Increasing protein phosphorylation/activation is indicated in red. Blue indicates decreasing protein phosphorylation/activation. Abbreviations: Afa = afatinib, Tra = trastuzumab, untr = untreated

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