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Fig. 3 | BMC Cancer

Fig. 3

From: Determining the effects of trastuzumab, cetuximab and afatinib by phosphoprotein, gene expression and phenotypic analysis in gastric cancer cell lines

Fig. 3

Effects of trastuzumab and afatinib on kinase phosphorylation in MKN1 cells. Luminex analysis was performed to detect the effects on protein tyrosine kinases in MKN1 cells induced by trastuzumab and/or afatinib. Cells were treated for 3, 5, 15, 30, 60 and 240 min with 0.5 μM afatinib, 5 μg/ml trastuzumab or the combination of both. In the batch-corrected cluster analysis, the x-fold change of each activated protein is shown. Samples were clustered based on to the similarity of the activated proteins and treatment conditions. Significant effects between different treatment conditions are indicated by (*) with increasing size (0.01 < p-value ≤0.05, 0.001 < p-value ≤0.01 and p-value ≤0.001). Increasing protein phosphorylation/activation is indicated in red. Blue indicates decreasing protein phosphorylation/activation. Abbreviations: Afa = afatinib, Tra = trastuzumab, untr = untreated

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